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Abstract Due to its inbuilt ability to release biocompatible materials encapsulating living cells in a predefined location, 3D bioprinting is a promising technique for regenerating patient-specific tissues and organs. Among various 3D bioprinting techniques, extrusion-based 3D bio-printing ensures a higher percentage of cell release, ensuring suitable external and internal scaffold architectures. Scaffold architecture is mainly defined by filament geometry and width. A systematic selection of a set of process parameters, such as nozzle diameter, print speed, print distance, extrusion pressure, and material viscosity, can control the filament geometry and width, eventually confirming the user-defined scaffold porosity. For example, carefully selecting two sets of process parameters can result in a similar filament width. However, the lack of availability of sufficient analytical relations between printing process parameters and filament width creates a barrier to achieving defined scaffold architectures with available resources. In this paper, filament width was determined using an image processing technique and an analytical relationship was developed, including various process parameters to maintain defined filament width variation for different hydrogels within an acceptable range to confirm the overall geometric fidelity of the scaffold. Proposed analytical relations can help achieve defined scaffold architectures with available resources.